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addgene plasmid  (Addgene inc)


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    Structured Review

    Addgene inc addgene plasmid
    Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv%CE%B2+p300+dy+myc/pCMV%CE%B2-p300%2EDY-myc+(Plasmid+%2330490)/pmc12357098-50-9-9
    Average 93 stars, based on 3 article reviews
    addgene plasmid - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Mitochondrial hyper-acetylation induced by an engineered acetyltransferase promotes cellular senescence
    Article Snippet: pCMVβ-p300-myc , a gift from Tso-Pang Yao , Addgene Plasmid #30489. .. pCMVβ-p300.DY-myc , a gift from Dr. Tso-Pang Yao , Addgene Plasmid #30490. ..

    Article Title: Inactivation of nuclear histone deacetylases by EP300 disrupts the MiCEE complex in idiopathic pulmonary fibrosis
    Article Snippet: .. For expression of dnEP300 we used pCMVβ-p300.DY-myc, which was a gift from Tso-Pang Yao (Addgene plasmid #30490; http://n2t.net/addgene:30490 ; RRID:Addgene_30490). .. Cells were treated with EZH2 inhibitor UNC-1999 (Sigma, #SML0778) at 2 μM final concentration for 24 h. The solvent used for dissolving these inhibitors, following manufacturer’s instruction, were used as a negative control.

    Expressing:

    Article Title: Inactivation of nuclear histone deacetylases by EP300 disrupts the MiCEE complex in idiopathic pulmonary fibrosis
    Article Snippet: .. For expression of dnEP300 we used pCMVβ-p300.DY-myc, which was a gift from Tso-Pang Yao (Addgene plasmid #30490; http://n2t.net/addgene:30490 ; RRID:Addgene_30490). .. Cells were treated with EZH2 inhibitor UNC-1999 (Sigma, #SML0778) at 2 μM final concentration for 24 h. The solvent used for dissolving these inhibitors, following manufacturer’s instruction, were used as a negative control.

    Construct:

    Article Title: Mitochondrial hyper-acetylation induced by an engineered acetyltransferase promotes cellular senescence
    Article Snippet: SIRT3 (D22A3) Rabbit mAb (Cell signaling, #5490), COXIV mouse mAb (Cell Signaling clone#4D11-B3-E8, #11967), VDAC (D73D12) Rabbit mAb (Cell Signaling, #4661), HSP60 (D307) Antibody (Cell Signaling, #4870), ETFB Polyclonal antibody (Proteintech, #17925-1-AP), anti-α-tubulin antibody (clone B-5-1-2, Sigma), monoclonal anti-FLAG M2-Pertoxidase (HRP) antibody (Merck, #A8592), anti-HA antibody (clone 3F10, Roche), V5-tag Polyclonal antibody (Proteintech, #14440-1-AP). anti-FLAG M2 Affinity Gel (Merck, #A2220) was used for immunoprecipitation. .. Plasmids for eMAT were constructed from human p300 cDNA fragments amplified from pCMVβ-p300-myc (addgene#30489), or pCMVβ-p300.DY-myc (addgene#30490) for the catalytic mutant. .. The amplified catalytic cDNA fragment (p300_HAT) with 5′-AgeI site was cloned into EcoRI and NotI sites of pcDNA3 vector (Invitrogen) with c-terminal FLAG tag or V5 tag sequence.

    Amplification:

    Article Title: Mitochondrial hyper-acetylation induced by an engineered acetyltransferase promotes cellular senescence
    Article Snippet: SIRT3 (D22A3) Rabbit mAb (Cell signaling, #5490), COXIV mouse mAb (Cell Signaling clone#4D11-B3-E8, #11967), VDAC (D73D12) Rabbit mAb (Cell Signaling, #4661), HSP60 (D307) Antibody (Cell Signaling, #4870), ETFB Polyclonal antibody (Proteintech, #17925-1-AP), anti-α-tubulin antibody (clone B-5-1-2, Sigma), monoclonal anti-FLAG M2-Pertoxidase (HRP) antibody (Merck, #A8592), anti-HA antibody (clone 3F10, Roche), V5-tag Polyclonal antibody (Proteintech, #14440-1-AP). anti-FLAG M2 Affinity Gel (Merck, #A2220) was used for immunoprecipitation. .. Plasmids for eMAT were constructed from human p300 cDNA fragments amplified from pCMVβ-p300-myc (addgene#30489), or pCMVβ-p300.DY-myc (addgene#30490) for the catalytic mutant. .. The amplified catalytic cDNA fragment (p300_HAT) with 5′-AgeI site was cloned into EcoRI and NotI sites of pcDNA3 vector (Invitrogen) with c-terminal FLAG tag or V5 tag sequence.

    Mutagenesis:

    Article Title: Mitochondrial hyper-acetylation induced by an engineered acetyltransferase promotes cellular senescence
    Article Snippet: SIRT3 (D22A3) Rabbit mAb (Cell signaling, #5490), COXIV mouse mAb (Cell Signaling clone#4D11-B3-E8, #11967), VDAC (D73D12) Rabbit mAb (Cell Signaling, #4661), HSP60 (D307) Antibody (Cell Signaling, #4870), ETFB Polyclonal antibody (Proteintech, #17925-1-AP), anti-α-tubulin antibody (clone B-5-1-2, Sigma), monoclonal anti-FLAG M2-Pertoxidase (HRP) antibody (Merck, #A8592), anti-HA antibody (clone 3F10, Roche), V5-tag Polyclonal antibody (Proteintech, #14440-1-AP). anti-FLAG M2 Affinity Gel (Merck, #A2220) was used for immunoprecipitation. .. Plasmids for eMAT were constructed from human p300 cDNA fragments amplified from pCMVβ-p300-myc (addgene#30489), or pCMVβ-p300.DY-myc (addgene#30490) for the catalytic mutant. .. The amplified catalytic cDNA fragment (p300_HAT) with 5′-AgeI site was cloned into EcoRI and NotI sites of pcDNA3 vector (Invitrogen) with c-terminal FLAG tag or V5 tag sequence.

    other:

    Article Title: DNA polymerase ι is acetylated in response to S N 2 alkylating agents
    Article Snippet: pCMVβ-p300.DY-myc , , , gift from Tso-Pang Yao (Addgene # 30489).



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    addgene plasmid  (Addgene inc)
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    https://www.bioz.com/product/pcmv%CE%B2+p300+dy+myc/pCMV%CE%B2-p300%2EDY-myc+(Plasmid+%2330490)/pmc12357098-50-9-9
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    pcmvβ p300 dy myc  (Addgene inc)
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    Design and characterization of engineered mitochondrial acetyltransferase; eMAT (A) Schematic structure of eMAT. Mitochondria targeting sequence (MTS) of PDHA1, COX4, NDUFS1, SIRT4, and CAMP were fused with <t>p300</t> HAT domain (1282–1667 a.a.) or Core domain (965–1810 a.a.). (B) Subcellular localization of the fusion constructs (anti-FLAG, colored in green). PDHA1-HAT and COX4-HAT merged with a mitochondria marker protein ETFβ (colored in red). The blue signal indicates DAPI (nuclear marker). Box with magenta; constructs that showed mitochondrial localization. Scale bar: 10 μm. (C) Immunoblot images of total cell lysates from HEK293T transfected with indicated constructs. Top; anti-AcK antibody, Middle; anti-FLAG antibody, Bottom; anti-α-tubulin antibody as a loading control. (D) Immunoblot analysis of protein acetylation in mitochondria. Mitochondria were isolated from HEK293T cells transfected with indicated constructs, and immunoblot analysis was performed with anti-AcK (top), anti-FLAG (middle), and anti-ETFβ as mitochondria loading control (bottom). (E) Structure of eMAT. Top; schematic amino acid sequence of eMAT. Bottom; Structure of eMAT protein predicted with AlphaFold2 based ColabFold program (v1.5.3).
    Pcmvβ P300 Dy Myc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv%CE%B2+p300+dy+myc/pCMV%CE%B2-p300%2EDY-myc+(Plasmid+%2330490)/pmc12357098-383-15-16
    Average 93 stars, based on 1 article reviews
    pcmvβ p300 dy myc - by Bioz Stars, 2026-10
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    tso pang yao  (Addgene inc)
    93
    Addgene inc tso pang yao
    Design and characterization of engineered mitochondrial acetyltransferase; eMAT (A) Schematic structure of eMAT. Mitochondria targeting sequence (MTS) of PDHA1, COX4, NDUFS1, SIRT4, and CAMP were fused with <t>p300</t> HAT domain (1282–1667 a.a.) or Core domain (965–1810 a.a.). (B) Subcellular localization of the fusion constructs (anti-FLAG, colored in green). PDHA1-HAT and COX4-HAT merged with a mitochondria marker protein ETFβ (colored in red). The blue signal indicates DAPI (nuclear marker). Box with magenta; constructs that showed mitochondrial localization. Scale bar: 10 μm. (C) Immunoblot images of total cell lysates from HEK293T transfected with indicated constructs. Top; anti-AcK antibody, Middle; anti-FLAG antibody, Bottom; anti-α-tubulin antibody as a loading control. (D) Immunoblot analysis of protein acetylation in mitochondria. Mitochondria were isolated from HEK293T cells transfected with indicated constructs, and immunoblot analysis was performed with anti-AcK (top), anti-FLAG (middle), and anti-ETFβ as mitochondria loading control (bottom). (E) Structure of eMAT. Top; schematic amino acid sequence of eMAT. Bottom; Structure of eMAT protein predicted with AlphaFold2 based ColabFold program (v1.5.3).
    Tso Pang Yao, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv%CE%B2+p300+dy+myc/pCMV%CE%B2-p300%2EDY-myc+(Plasmid+%2330490)/pmc06527704-253-12-14
    Average 93 stars, based on 1 article reviews
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    Image Search Results


    Design and characterization of engineered mitochondrial acetyltransferase; eMAT (A) Schematic structure of eMAT. Mitochondria targeting sequence (MTS) of PDHA1, COX4, NDUFS1, SIRT4, and CAMP were fused with p300 HAT domain (1282–1667 a.a.) or Core domain (965–1810 a.a.). (B) Subcellular localization of the fusion constructs (anti-FLAG, colored in green). PDHA1-HAT and COX4-HAT merged with a mitochondria marker protein ETFβ (colored in red). The blue signal indicates DAPI (nuclear marker). Box with magenta; constructs that showed mitochondrial localization. Scale bar: 10 μm. (C) Immunoblot images of total cell lysates from HEK293T transfected with indicated constructs. Top; anti-AcK antibody, Middle; anti-FLAG antibody, Bottom; anti-α-tubulin antibody as a loading control. (D) Immunoblot analysis of protein acetylation in mitochondria. Mitochondria were isolated from HEK293T cells transfected with indicated constructs, and immunoblot analysis was performed with anti-AcK (top), anti-FLAG (middle), and anti-ETFβ as mitochondria loading control (bottom). (E) Structure of eMAT. Top; schematic amino acid sequence of eMAT. Bottom; Structure of eMAT protein predicted with AlphaFold2 based ColabFold program (v1.5.3).

    Journal: iScience

    Article Title: Mitochondrial hyper-acetylation induced by an engineered acetyltransferase promotes cellular senescence

    doi: 10.1016/j.isci.2025.113233

    Figure Lengend Snippet: Design and characterization of engineered mitochondrial acetyltransferase; eMAT (A) Schematic structure of eMAT. Mitochondria targeting sequence (MTS) of PDHA1, COX4, NDUFS1, SIRT4, and CAMP were fused with p300 HAT domain (1282–1667 a.a.) or Core domain (965–1810 a.a.). (B) Subcellular localization of the fusion constructs (anti-FLAG, colored in green). PDHA1-HAT and COX4-HAT merged with a mitochondria marker protein ETFβ (colored in red). The blue signal indicates DAPI (nuclear marker). Box with magenta; constructs that showed mitochondrial localization. Scale bar: 10 μm. (C) Immunoblot images of total cell lysates from HEK293T transfected with indicated constructs. Top; anti-AcK antibody, Middle; anti-FLAG antibody, Bottom; anti-α-tubulin antibody as a loading control. (D) Immunoblot analysis of protein acetylation in mitochondria. Mitochondria were isolated from HEK293T cells transfected with indicated constructs, and immunoblot analysis was performed with anti-AcK (top), anti-FLAG (middle), and anti-ETFβ as mitochondria loading control (bottom). (E) Structure of eMAT. Top; schematic amino acid sequence of eMAT. Bottom; Structure of eMAT protein predicted with AlphaFold2 based ColabFold program (v1.5.3).

    Article Snippet: Plasmids for eMAT were constructed from human p300 cDNA fragments amplified from pCMVβ-p300-myc (addgene#30489), or pCMVβ-p300.DY-myc (addgene#30490) for the catalytic mutant.

    Techniques: Sequencing, Construct, Marker, Western Blot, Transfection, Control, Isolation